Journal: Arthritis Research & Therapy

Article Title: Fucosyltransferase 1 mediates angiogenesis, cell adhesion and rheumatoid arthritis synovial tissue fibroblast proliferation

doi: 10.1186/ar4456

Figure Lengend Snippet: α(1,2)-linked fucosylated proteins are expressed in rheumatoid arthritis (RA). (A) RA synovial tissue (ST) homogenates contained more α(1,2)-linked fucosylated proteins than did osteoarthritis (OA) or normal (NL) STs (normalized to total protein concentration). Results are expressed as a ratio of the amount of fucosylated BSA/total proteins in the ST homogenates using BSA as a standard. (B) Photographs of RA ST. The far left panel shows staining with mouse anti-collagen 1. The middle panel shows staining with Ulex Europeaus Agglutinin 1 lectin (UEA-1) and goat anti-UEA-1. The right panel shows merging of the left panel and middle panel. Yellow indicates α(1,2)-linked fucosylated proteins associated with RA ST fibroblasts and the blue indicates DAPI staining of the tissue (original magnification 100×). (C) α(1,2)-linked fucosylated proteins in RA synovial fibroblast-conditioned medium were significantly higher than in OA and NL synovial fibroblast-conditioned medium. (D) α(1,2)-linked fucosylated proteins in RA synovial fibroblast cell lysates were significantly higher than in NL synovial fibroblast cultures: 2′fucosyllactose-bovine serum albumin (2′FL-BSA) was used as a standard.

Article Snippet: UEA-1, 2 μg/ml (Vector laboratories Inc, Burlingame, CA, USA) was added for 90 minutes followed by 10 μg/ml biotinylated goat anti-UEA-1 antibody (Vector laboratories Inc) for 60 minutes, then streptavidin-horseradish peroxidase (HRP) for 30 minutes.

Techniques: Protein Concentration, Staining

Journal: Cell

Article Title: Tunable expression tools enable single-cell strain distinction in the gut microbiome

doi: 10.1016/j.cell.2017.03.041

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Fluorescein Labeled Ulex Europaeus Agglutinin I , Vector Labs , Cat#H-1000.

Techniques: Recombinant, Purification, Labeling, Plasmid Preparation, Luciferase, Mouse Assay, Software

Journal: International journal of molecular sciences

Article Title: An Improved Method for Cell Type-Selective Glycomic Analysis of Tissue Sections Assisted by Fluorescence Laser Microdissection.

doi: 10.3390/ijms20030700

Figure Lengend Snippet: Figure 1. Comparison of the current and new methods for laser microdissection–lectin microarray (LMD-LMA) analysis. (A) Comparison of tissue dissection. In Method 1, tissue fragments were collected from hematoxylin-stained formalin-fixed paraffin-embedded (FFPE) tissue sections based on morphological observation by LMD. For cell type-selective collection, Method 2 employed two serial sections, where one was stained with a cell type-specific probe for observation and one with hematoxylin for tissue dissection by LMD. In contrast to these current methods, in the new method called Method 3, tissue fragments were collected directly from the section that was fluorescently stained with a cell type-specific probe under fluorescent observation. This fluorescence LMD allowed for a more accurate and reproducible tissue collection. The procedure after the tissue dissection for LMA analysis was the same in these three methods. (B) Schematic overview of LMD-LMA analysis of hematoxylin- and fluorescent-stained tissue sections.

Article Snippet: For fluorescent double staining, after blocking as described above, the sections were serially incubated with a biotinylated probe (5 μg/mL in PBS; PNA, anti-CK5 antibody, and DBA from Vector Laboratories) for 5 h at RT, a fluorescent isothiocyanate-labeled lectin (5 μg/mL in PBS; UEA-I, PNA, DBA from Vector Laboratories) overnight at 4 ◦C, and Alexa Fluor 647-conjugated streptavidin (1 μg/mL in PBS; Invitrogen), and then mounted in ProLong Gold antifade reagent (Invitrogen).

Techniques: Comparison, Laser Capture Microdissection, Microarray, Dissection, Staining